Neuropeptide Y Y1 receptor in human dental pulp cells of noncarious and carious teeth

IA El Karim, PJ Lamey, GJ Linden… - International …, 2008 - Wiley Online Library
IA El Karim, PJ Lamey, GJ Linden, FT Lundy
International endodontic journal, 2008Wiley Online Library
Aim To determine the distribution of the NPY Y1 receptor in carious and noncarious human
dental pulp tissue using immunohistochemistry. A subsidiary aim was to confirm the
presence of the NPY Y1 protein product in membrane fractions of dental pulp tissue from
carious and noncarious teeth using western blotting. Methodology Twenty two dental pulp
samples were collected from carious and noncarious extracted teeth. Ten samples were
processed for immunohistochemistry using a specific antibody to the NPY Y1 receptor …
Abstract
Aim  To determine the distribution of the NPY Y1 receptor in carious and noncarious human dental pulp tissue using immunohistochemistry. A subsidiary aim was to confirm the presence of the NPY Y1 protein product in membrane fractions of dental pulp tissue from carious and noncarious teeth using western blotting.
Methodology  Twenty two dental pulp samples were collected from carious and noncarious extracted teeth. Ten samples were processed for immunohistochemistry using a specific antibody to the NPY Y1 receptor. Twelve samples were used to obtain membrane extracts which were electrophoresed, blotted onto nitrocellulose and probed with NPY Y1 receptor antibody. Kruskal–Wallis one‐way analysis of variance was employed to test for overall statistical differences between NPY Y1 levels in noncarious, moderately carious and grossly carious teeth.
Results  Neuropeptide Y Y1 receptor immunoreactivity was detected on the walls of blood vessels in pulp tissue from noncarious teeth. In carious teeth NPY Y1 immunoreactvity was observed on nerve fibres, blood vessels and inflammatory cells. Western blotting indicated the presence and confirmed the variability of NPY Y1 receptor protein expression in solubilised membrane preparations of human dental pulp tissue from carious and noncarious teeth.
Conclusions  Neuropeptide Y Y1 is expressed in human dental pulp tissue with evidence of increased expression in carious compared with noncarious teeth, suggesting a role for NPY Y1 in modulation of caries induced pulpal inflammation.
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