Simultaneous assessment of NF-κB/p65 phosphorylation and nuclear localization using imaging flow cytometry
O Maguire, K O'Loughlin, H Minderman - Journal of immunological …, 2015 - Elsevier
Abstract Aberrant activity of Nuclear Factor-kappaB (NF-κB) is associated with many
diseases and is therapeutically targeted. Post-translational modifications, particularly
phosphorylation of the RELA/p65 sub-unit, are essential for cytoplasmic to nuclear
localization of NF-κB/p65 and initiation of transcription of downstream target genes.
Immunoblot and phospho-flow cytometry have been used to study the relationship between
phosphorylation motifs and NF-κB activation and microscopic analysis of nuclear …
diseases and is therapeutically targeted. Post-translational modifications, particularly
phosphorylation of the RELA/p65 sub-unit, are essential for cytoplasmic to nuclear
localization of NF-κB/p65 and initiation of transcription of downstream target genes.
Immunoblot and phospho-flow cytometry have been used to study the relationship between
phosphorylation motifs and NF-κB activation and microscopic analysis of nuclear …
