Novel effect of glucagon-like peptide-2 for hepatocellular injury in a parenterally fed rat model of short bowel syndrome
K Yano, T Kaji, S Onishi, S Machigashira… - Pediatric Surgery …, 2019 - Springer
K Yano, T Kaji, S Onishi, S Machigashira, T Nagai, T Harumatsu, K Yamada, W Yamada…
Pediatric Surgery International, 2019•SpringerPurpose Short bowel syndrome (SBS) patients require long-term parenteral nutrition
following massive bowel resection, which causes intestinal failure-associated liver disease
(IFALD). Previous reports have shown that glucagon-like peptide-2 (GLP-2) resulted in the
bowel adaptation for SBS. The aim of this study was to evaluate the effect of GLP-2 for IFALD
in a parenterally fed rat model. Methods Using rat, a catheter was placed in the jugular vein,
and 90% small bowel resection (SBR) was performed. Animals were divided into three …
following massive bowel resection, which causes intestinal failure-associated liver disease
(IFALD). Previous reports have shown that glucagon-like peptide-2 (GLP-2) resulted in the
bowel adaptation for SBS. The aim of this study was to evaluate the effect of GLP-2 for IFALD
in a parenterally fed rat model. Methods Using rat, a catheter was placed in the jugular vein,
and 90% small bowel resection (SBR) was performed. Animals were divided into three …
Purpose
Short bowel syndrome (SBS) patients require long-term parenteral nutrition following massive bowel resection, which causes intestinal failure-associated liver disease (IFALD). Previous reports have shown that glucagon-like peptide-2 (GLP-2) resulted in the bowel adaptation for SBS. The aim of this study was to evaluate the effect of GLP-2 for IFALD in a parenterally fed rat model.
Methods
Using rat, a catheter was placed in the jugular vein, and 90% small bowel resection (SBR) was performed. Animals were divided into three groups: SBR and total parenteral nutrition (TPN) (SBS/TPN group), SBR and TPN plus GLP-2 at 1 µg/kg/h [SBS/TPN/GLP-2 (low) group], and SBR and TPN plus GLP-2 at 10 µg/kg/h [SBS/TPN/GLP-2 (high) group]. On day 13, the liver was harvested and analyzed by using nonalcoholic fatty liver disease (NAFLD) score.
Results
Histologically, hepatic steatosis in the SBS/TPN group and SBS/TPN/GLP-2 (high) group was observed. Both steatosis and lobular inflammation score in the SBS/TPN/GLP-2 (low) group were significantly lower compared with those in the other two groups (p < 0.05). Active NAFLD score in the SBS/TPN/GLP-2 (low) group was significantly lower compared with that in the SBS/TPN/GLP-2 (high) group (p < 0.01).
Conclusion
Low-dose GLP-2 intravenous administration improves hepatic steatosis of IFALD following in an SBS parenterally fed rat model.
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