Isotopomer measurement techniques in metabolic flux analysis II: mass spectrometry
Mass spectrometry (MS) offers a sensitive, reliable, and highly accurate method for
measurement of isotopic labeling, which is required for generating comprehensive flux maps
using metabolic flux analysis (MFA). We present protocols for assessing isotope labeling in
a wide range of biochemical species, including proteinogenic amino acids, free organic and
amino acids, sugar phosphates, lipids, starch-glucose, and RNA-ribose. We describe the
steps of sample preparation, MS analysis, and data handling required to obtain high-quality …
measurement of isotopic labeling, which is required for generating comprehensive flux maps
using metabolic flux analysis (MFA). We present protocols for assessing isotope labeling in
a wide range of biochemical species, including proteinogenic amino acids, free organic and
amino acids, sugar phosphates, lipids, starch-glucose, and RNA-ribose. We describe the
steps of sample preparation, MS analysis, and data handling required to obtain high-quality …
Abstract
Mass spectrometry (MS) offers a sensitive, reliable, and highly accurate method for measurement of isotopic labeling, which is required for generating comprehensive flux maps using metabolic flux analysis (MFA). We present protocols for assessing isotope labeling in a wide range of biochemical species, including proteinogenic amino acids, free organic and amino acids, sugar phosphates, lipids, starch-glucose, and RNA-ribose. We describe the steps of sample preparation, MS analysis, and data handling required to obtain high-quality isotope labeling measurements that are applicable to MFA. By selecting target analytes that maximize identifiability of the key fluxes of interest, MS measurements of isotope labeling can provide a powerful platform for assessing metabolic fluxes in complex biochemical networks.
Springer