MyriMatch: highly accurate tandem mass spectral peptide identification by multivariate hypergeometric analysis

DL Tabb, CG Fernando… - Journal of proteome …, 2007 - ACS Publications
DL Tabb, CG Fernando, MC Chambers
Journal of proteome research, 2007ACS Publications
Shotgun proteomics experiments are dependent upon database search engines to identify
peptides from tandem mass spectra. Many of these algorithms score potential identifications
by evaluating the number of fragment ions matched between each peptide sequence and an
observed spectrum. These systems, however, generally do not distinguish between
matching an intense peak and matching a minor peak. We have developed a statistical
model to score peptide matches that is based upon the multivariate hypergeometric …
Shotgun proteomics experiments are dependent upon database search engines to identify peptides from tandem mass spectra. Many of these algorithms score potential identifications by evaluating the number of fragment ions matched between each peptide sequence and an observed spectrum. These systems, however, generally do not distinguish between matching an intense peak and matching a minor peak. We have developed a statistical model to score peptide matches that is based upon the multivariate hypergeometric distribution. This scorer, part of the “MyriMatch” database search engine, places greater emphasis on matching intense peaks. The probability that the best match for each spectrum has occurred by random chance can be employed to separate correct matches from random ones. We evaluated this software on data sets from three different laboratories employing three different ion trap instruments. Employing a novel system for testing discrimination, we demonstrate that stratifying peaks into multiple intensity classes improves the discrimination of scoring. We compare MyriMatch results to those of Sequest and X!Tandem, revealing that it is capable of higher discrimination than either of these algorithms. When minimal peak filtering is employed, performance plummets for a scoring model that does not stratify matched peaks by intensity. On the other hand, we find that MyriMatch discrimination improves as more peaks are retained in each spectrum. MyriMatch also scales well to tandem mass spectra from high-resolution mass analyzers. These findings may indicate limitations for existing database search scorers that count matched peaks without differentiating them by intensity. This software and source code is available under Mozilla Public License at this URL:  http://www.mc.vanderbilt.edu/msrc/bioinformatics/.
Keywords: proteomics • identification • statistical distribution • reversed database • peak filtering
ACS Publications