[HTML][HTML] Fatty acid labeling from glutamine in hypoxia can be explained by isotope exchange without net reductive isocitrate dehydrogenase (IDH) flux
Acetyl-CoA is an important anabolic precursor for lipid biosynthesis. In the conventional view
of mammalian metabolism, acetyl-CoA is primarily derived by the oxidation of glucose-
derived pyruvate in mitochondria. Recent studies have employed isotope tracers to show
that in cancer cells grown in hypoxia or with defective mitochondria, a major fraction of acetyl-
CoA is produced via another route, reductive carboxylation of glutamine-derived α-
ketoglutarate (catalyzed by reverse flux through isocitrate dehydrogenase, IDH). Here, we …
of mammalian metabolism, acetyl-CoA is primarily derived by the oxidation of glucose-
derived pyruvate in mitochondria. Recent studies have employed isotope tracers to show
that in cancer cells grown in hypoxia or with defective mitochondria, a major fraction of acetyl-
CoA is produced via another route, reductive carboxylation of glutamine-derived α-
ketoglutarate (catalyzed by reverse flux through isocitrate dehydrogenase, IDH). Here, we …