[HTML][HTML] hESC-derived Olig2+ progenitors generate a subtype of astroglia with protective effects against ischaemic brain injury

P Jiang, C Chen, R Wang, OV Chechneva… - Nature …, 2013 - nature.com
P Jiang, C Chen, R Wang, OV Chechneva, SH Chung, MS Rao, DE Pleasure, Y Liu
Nature communications, 2013nature.com
Human pluripotent stem cells (hPSCs) have been differentiated to astroglia, but the
utilization of hPSC-derived astroglia as cell therapy for neurological diseases has not been
well studied. Astroglia are heterogeneous, and not all astroglia are equivalent in promoting
neural repair. A prerequisite for cell therapy is to derive defined cell populations with
superior therapeutic effects. Here we use an Olig2-GFP human embryonic stem cell (hESC)
reporter to demonstrate that hESC-derived Olig2+ progenitors generate a subtype of …
Abstract
Human pluripotent stem cells (hPSCs) have been differentiated to astroglia, but the utilization of hPSC-derived astroglia as cell therapy for neurological diseases has not been well studied. Astroglia are heterogeneous, and not all astroglia are equivalent in promoting neural repair. A prerequisite for cell therapy is to derive defined cell populations with superior therapeutic effects. Here we use an Olig2-GFP human embryonic stem cell (hESC) reporter to demonstrate that hESC-derived Olig2+ progenitors generate a subtype of previously uncharacterized astroglia (Olig2PC-Astros). These Olig2PC-Astros differ substantially from astroglia differentiated from Olig2-negative hESC-derived neural progenitor cells (NPC-Astros), particularly in their neuroprotective properties. When grafted into brains subjected to global ischaemia, Olig2PC-Astros exhibit superior neuroprotective effects and improved behavioural outcome compared to NPC-Astros. Thus, this new paradigm of human astroglial differentiation is useful for studying the heterogeneity of human astroglia, and the unique Olig2PC-Astros may constitute a new cell therapy for treating cerebral ischaemia and other neurological diseases.
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