Correlation of plasma viral loads and presence of Chikungunya IgM antibodies with cytokine/chemokine levels during acute Chikungunya virus infection
V Reddy, RS Mani, A Desai… - Journal of medical …, 2014 - Wiley Online Library
V Reddy, RS Mani, A Desai, V Ravi
Journal of medical virology, 2014•Wiley Online LibraryChikungunya (CHIKV) is an emerging arboviral infection of public health concern in India
contributing to widespread morbidity. The precise molecular events occurring early in the
infection have not been well understood. Cytokines/chemokines are suspected to play a key
role in its pathogenesis. Very few studies have correlated the plasma levels of
cytokines/chemokines with diagnostic markers such as viral loads and presence of CHIKV
IgM antibodies. Understanding these dynamics in the early phase of CHIKV infection is likely …
contributing to widespread morbidity. The precise molecular events occurring early in the
infection have not been well understood. Cytokines/chemokines are suspected to play a key
role in its pathogenesis. Very few studies have correlated the plasma levels of
cytokines/chemokines with diagnostic markers such as viral loads and presence of CHIKV
IgM antibodies. Understanding these dynamics in the early phase of CHIKV infection is likely …
Chikungunya (CHIKV) is an emerging arboviral infection of public health concern in India contributing to widespread morbidity. The precise molecular events occurring early in the infection have not been well understood. Cytokines/chemokines are suspected to play a key role in its pathogenesis. Very few studies have correlated the plasma levels of cytokines/chemokines with diagnostic markers such as viral loads and presence of CHIKV IgM antibodies. Understanding these dynamics in the early phase of CHIKV infection is likely to provide an insight into the evolution of the immune response, identify biomarkers for assessing severity, and for development of newer therapeutic strategies. This study was therefore undertaken to estimate the levels of various cytokines/chemokines in plasma samples of patients infected with CHIKV and correlate to viral load and CHIKV IgM antibodies. Cytokine/chemokine levels and viral loads in plasma were measured using cytometric bead array and TaqMan real time PCR assay, respectively. The findings revealed that acute phase of CHIKV infection is characterized by predominant inflammatory responses mediated by IL‐6, IL‐8, IP‐10, MCP‐1, and MIG (P < 0.003). Plasma levels of IL‐6 (r = 0.53, P < 0.05) and MCP‐1 (r = 0.83, P < 0.05) emerged as reliable biomarkers of high viral loads in Chikungunya patients. Further, presence of elevated levels of MCP‐1 and MIG during the chronic phase of the disease suggests that these chemokines may contribute to perpetuation of symptoms. Hence, these chemokines might serve as targets for the development of treatment to ameliorate the symptoms during the acute phase and prevent the development of chronic manifestations. J. Med. Virol. 86:1393–1401, 2014. © 2014 Wiley Periodicals, Inc.
Wiley Online Library