Coproporphyrinogen oxidase, the sixth enzyme in the biosynthetic heme pathway, catalyzes
the oxidative decarboxylation of coproporphyrinogen III to protoporphyrinogen IX. A
reversed-phase high pressure liquid chromatography method was developed to measure
coproporphyrinogen oxidase enzymatic activity in rat liver. With this method, the separation,
identification and quantification of coproporphyrin III (oxidized substrate) and protoporphyrin
IX (oxidized product) present in the assays could be carried out with no need of …