Raman microscopy for dynamic molecular imaging of living cells
Journal of biomedical optics, 2008•spiedigitallibrary.org
We demonstrate dynamic imaging of molecular distribution in unstained living cells using
Raman scattering. By combining slit-scanning detection and optimizing the excitation
wavelength, we imaged the dynamic molecular distributions of cytochrome c, protein beta
sheets, and lipids in unstained HeLa cells with a temporal resolution of 3 minutes. We found
that 532-nm excitation can be used to generate strong Raman scattering signals and to
suppress autofluorescence that typically obscures Raman signals. With this technique, we …
Raman scattering. By combining slit-scanning detection and optimizing the excitation
wavelength, we imaged the dynamic molecular distributions of cytochrome c, protein beta
sheets, and lipids in unstained HeLa cells with a temporal resolution of 3 minutes. We found
that 532-nm excitation can be used to generate strong Raman scattering signals and to
suppress autofluorescence that typically obscures Raman signals. With this technique, we …
We demonstrate dynamic imaging of molecular distribution in unstained living cells using Raman scattering. By combining slit-scanning detection and optimizing the excitation wavelength, we imaged the dynamic molecular distributions of cytochrome c, protein beta sheets, and lipids in unstained HeLa cells with a temporal resolution of . We found that excitation can be used to generate strong Raman scattering signals and to suppress autofluorescence that typically obscures Raman signals. With this technique, we reveal time-resolved distributions of cytochrome c and other biomolecules in living cells in the process of cytokinesis without the need for fluorescent labels or markers.
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