Cytidine triphosphate (CTP) synthetase activity during cell cycle progression in normal and malignant T-lymphocytic cells
AA Van Den Berg, H Van Lenthe, JBA Kipp… - European Journal of …, 1995 - Elsevier
AA Van Den Berg, H Van Lenthe, JBA Kipp, D De Korte, ABP Van Kuilenburg…
European Journal of Cancer, 1995•ElsevierThe role of cytidine triphosphate (CTP) synthetase (EC 6.3. 4.2.) m the pyrimidine
ribonucleotide metabolism of MOLT-3 human T-ALL cell line cells and normal human T
lymphocytes during the cell cycle traverse was studied. Highly pure G1-phase samples and
samples enriched in S-phase cells were obtained by counterflow centrifugation. The activity
of CTP synthetase in situ, measured in pulse-chase experiments, was similar in the G1-
phase and S-phase MOLT-3 cells. In contrast, in S-phase T lymphocytes, an increased …
ribonucleotide metabolism of MOLT-3 human T-ALL cell line cells and normal human T
lymphocytes during the cell cycle traverse was studied. Highly pure G1-phase samples and
samples enriched in S-phase cells were obtained by counterflow centrifugation. The activity
of CTP synthetase in situ, measured in pulse-chase experiments, was similar in the G1-
phase and S-phase MOLT-3 cells. In contrast, in S-phase T lymphocytes, an increased …
The role of cytidine triphosphate (CTP) synthetase (EC 6.3.4.2.) m the pyrimidine ribonucleotide metabolism of MOLT-3 human T-ALL cell line cells and normal human T lymphocytes during the cell cycle traverse was studied. Highly pure G1-phase samples and samples enriched in S-phase cells were obtained by counterflow centrifugation. The activity of CTP synthetase in situ, measured in pulse-chase experiments, was similar in the G1-phase and S-phase MOLT-3 cells. In contrast, in S-phase T lymphocytes, an increased activity of CTP synthetase was observed compared with G1-phase T lymphocytes. Nevertheless, the MOLT-3 samples showed an increased activity of CTP synthetase in comparison with either G1-phase or S-phase enriched samples of normal T lymphocytes. Therefore, the increased activity of CTP synthetase of MOLT-3 cells is a cell cycleindependent feature, whereas among normal T lymphocytes, the increase in activity of CTP synthetase that arises after a growth stimulus is more prominent in the S-phase.
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