[HTML][HTML] Regulation of human cytidine triphosphate synthetase 2 by phosphorylation
KM Kassel, MJ Higgins, M Hines, LM Graves - Journal of biological …, 2010 - ASBMB
Cytidine triphosphate synthetase (CTPS) is the rate-limiting enzyme in de novo CTP
synthesis and is required for the formation of RNA, DNA, and phospholipids. This study
determined the kinetic properties of the individual human CTPS isozymes (hCTPS1 and
hCTPS2) and regulation through substrate concentration, oligomerization, and
phosphorylation. Kinetic analysis demonstrated that both hCTPS1 and hCTPS2 were
maximally active at physiological concentrations of ATP, GTP, and glutamine, whereas the K …
synthesis and is required for the formation of RNA, DNA, and phospholipids. This study
determined the kinetic properties of the individual human CTPS isozymes (hCTPS1 and
hCTPS2) and regulation through substrate concentration, oligomerization, and
phosphorylation. Kinetic analysis demonstrated that both hCTPS1 and hCTPS2 were
maximally active at physiological concentrations of ATP, GTP, and glutamine, whereas the K …