Background: Although humans require dietary choline for methyl donation, membrane
function, and neurotransmission, choline can also be derived from the de novo synthesis of
phosphatidylcholine, which is up-regulated by estrogen. A recommended Adequate Intake
(AI) exists for choline; however, an Estimated Average Requirement has not been set
because of a lack of sufficient human data. Objective: The objective of the study was to
evaluate the dietary requirements for choline in healthy men and women and to investigate …