Estimating energy expenditure in mice using an energy balance technique
International journal of obesity, 2013•nature.com
Objective: To compare, in mice, the accuracy of estimates of energy expenditure (EE) using
an energy balance technique (TEE bal: food energy intake and body composition change)
vs indirect calorimetry (TEE IC). Subjects: In 32 male C57BL/6J mice, EE was estimated
using an energy balance (caloric intake minus change in body energy stores) method over a
37-day period. EE was also measured in the same animals by indirect calorimetry. These
measures were compared. Results: The two methods were highly correlated (r 2= 0.87: TEE …
an energy balance technique (TEE bal: food energy intake and body composition change)
vs indirect calorimetry (TEE IC). Subjects: In 32 male C57BL/6J mice, EE was estimated
using an energy balance (caloric intake minus change in body energy stores) method over a
37-day period. EE was also measured in the same animals by indirect calorimetry. These
measures were compared. Results: The two methods were highly correlated (r 2= 0.87: TEE …
Abstract
Objective:
To compare, in mice, the accuracy of estimates of energy expenditure (EE) using an energy balance technique (TEE bal: food energy intake and body composition change) vs indirect calorimetry (TEE IC).
Subjects:
In 32 male C57BL/6J mice, EE was estimated using an energy balance (caloric intake minus change in body energy stores) method over a 37-day period. EE was also measured in the same animals by indirect calorimetry. These measures were compared.
Results:
The two methods were highly correlated (r 2= 0.87: TEE bal= 1.07* TEE IC–0.22, P< 0.0001). By Bland–Altman analysis, TEE bal estimates were slightly higher (4.6±1.5%; P< 0.05) than TEE IC estimates (Bias= 0.55 kcal per 24 h).
Conclusion:
TEE bal can be performed in ‘home cages’ and provides an accurate integrated long-term measurement of EE while minimizing potentially confounding stress that may accompany the use of indirect calorimetry systems. The technique can also be used to assess long-term energy intake.
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