Temporal expression profiling was utilized to define transcriptional regulatory pathways in
vivo in a mouse muscle regeneration model. Potential downstream targets of MyoD were
identified by temporal expression, promoter data base mining, and gel shift assays; Slug and
calpain 6 were identified as novel MyoD targets. Slug, a member of the snail/slug family of
zinc finger transcriptional repressors critical for mesoderm/ectoderm development, was
further shown to be a downstream target by using promoter/reporter constructs and …