Enhancement of lysine acetylation accelerates wound repair

F Spallotta, C Cencioni, S Straino… - … & integrative biology, 2013 - Taylor & Francis
F Spallotta, C Cencioni, S Straino, G Sbardella, S Castellano, MC Capogrossi, F Martelli
Communicative & integrative biology, 2013Taylor & Francis
In physiopathological conditions, such as diabetes, wound healing is significantly
compromised and chronic complications, including ulcers, may occur. In a mouse model of
skin repair, we recently reported that wound treatment with Sirtuin activators and class I
HDAC inhibitors induced keratinocyte proliferation and enhanced healing via a nitric oxide
(NO) dependent mechanism. We observed an increase in total protein acetylation in the
wound area, as determined by acetylation of α-tubulin and histone H3 Lysine 9. We …
In physiopathological conditions, such as diabetes, wound healing is significantly compromised and chronic complications, including ulcers, may occur. In a mouse model of skin repair, we recently reported that wound treatment with Sirtuin activators and class I HDAC inhibitors induced keratinocyte proliferation and enhanced healing via a nitric oxide (NO) dependent mechanism. We observed an increase in total protein acetylation in the wound area, as determined by acetylation of α-tubulin and histone H3 Lysine 9. We reasoned that this process activated cell function as well as regulated gene expression to foster tissue repair. We report here that the direct activation of P300/CBP-associated factor (PCAF) by the histone acetylase activator pentadecylidenemalonate 1b (SPV-106) induced Lysine acetylation in the wound area. This intervention was sufficient to enhance repair process by a NO-independent mechanism. Hence, an impairment of PCAF and/or other GCN5 family acetylases may delay skin repair in physiopathological conditions.
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