Studies of the residual glycogen branching enzyme activity present in human skin fibroblasts from patients with type IV glycogen storage disease
DH Brown, BI Brown - Biochemical and Biophysical Research …, 1983 - Elsevier
DH Brown, BI Brown
Biochemical and Biophysical Research Communications, 1983•ElsevierHuman skin fibroblasts from patients with Type IV glycogen storage disease, in which there
is a demonstrable deficiency of glycogen branching enzyme, were shown to be able to
synthesize [14 C] glycogen containing [14 C] glucose at branch points when sonicates
containing endogenous glycogen synthase a were incubated with UDP [14 C] glucose. The
branch point content of the glycogen synthesized by the Type IV cells was essentially the
same as that formed by normal cells, but the total synthetic capacity of the Type IV cells was …
is a demonstrable deficiency of glycogen branching enzyme, were shown to be able to
synthesize [14 C] glycogen containing [14 C] glucose at branch points when sonicates
containing endogenous glycogen synthase a were incubated with UDP [14 C] glucose. The
branch point content of the glycogen synthesized by the Type IV cells was essentially the
same as that formed by normal cells, but the total synthetic capacity of the Type IV cells was …
Human skin fibroblasts from patients with Type IV glycogen storage disease, in which there is a demonstrable deficiency of glycogen branching enzyme, were shown to be able to synthesize [14 C] glycogen containing [14 C] glucose at branch points when sonicates containing endogenous glycogen synthase a were incubated with UDP [14 C] glucose. The branch point content of the glycogen synthesized by the Type IV cells was essentially the same as that formed by normal cells, but the total synthetic capacity of the Type IV cells was lower. A new assay for the branching enzyme using glycogen synthase as the indicator enzyme has been developed. Using this assay it has been shown that the residual branching enzyme of affected children and of their heterozygote parents is less easily inhibited by an IgG antibody raised in rabbits against the normal human liver enzyme than is the branching enzyme of normal fibroblasts.
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