Annual influenza vaccination is recommended for immu-nocompromised patients. 1 2 Rituximab, an anti-CD20 monoclonal antibody, recently approved by American and European authorities for the treatment of rheumatoid arthritis (RA) patients failing tumour necrosis factor (TNF)-blocking agents, diminishes the number of circulating B cells for a period of six to nine months after infusion. 3 4 Circulating plasma cells and immunoglobulin levels are not affected by this therapy. Based on data in lymphoma patients, the package insert states that patients should not be vaccinated from one month before the administration of rituximab until six months after. 5 6

In those studies the underlying lymphoma and treatment with chemotherapy contributed to the diminished immunological response. The effect of rituximab on the outcome of influenza vaccination in RA is not known. We examined the humoral response upon influenza vaccination in four RA patients (three women, age range 55–61 years) treated with rituximab (1000 mg intravenously on days 0 and 14) combined with methotrexate (5–20 mg a week) and additional prednisone (5 mg) in one patient. Nineteen RA patients treated with TNF-blocking agents with or without disease-modifying antirheumatic drugs (79% women, mean age 56 years, range 40–71), and 20 healthy individuals (50% women, mean age 45 years, range 19–77) acted as controls. The three groups were well matched with respect to sex, age, prevaccination titres and previous influenza vaccination. Both patient groups had high disease activity scores (mean DAS28 3.47 and 4.44 for RA patients treated with rituximab and anti-TNF, respectively, p= 0.088, analysis of variance). Participants were vaccinated intramuscularly with a trivalent subunit vaccine (0.5 ml Influvac 2005–2006; Solvay, Weesp, the Netherlands). Haemagglutination-inhibition titres were measured just before vaccination and 28 days later, as described before. 7 8 Absolute lymphocyte counts were analysed using TruCOUNT tubes by flowcytometry. B cells were completely depleted (, 16106 cells/l) in all four patients from day 28 to day 84 after the first rituximab infusion. The vaccine was administered shortly after day 84, with only marginal B-cell reconstitution at the time of vaccination (median B-cell count, 106106 cells/l). As a result of low B-cell and patient numbers no trends could be determined in B-cell subsets. Even though only four RA patients treated with rituximab were evaluated, we found significantly lower postvaccination