In vivo analysis of gene expression in long-lived mice lacking the pregnancy-associated plasma protein A (PappA) gene
WR Swindell, MM Masternak, A Bartke - Experimental gerontology, 2010 - Elsevier
Mice lacking the pregnancy-associated plasma protein A (PappA) gene exhibit diminished
localized IGF-1 bioavailability and a 30% increase in mean life span. However, it is
uncertain which tissues exhibit reduced IGF-1 signals in the PappA (−/−) mouse, and
whether effects of this mutation parallel those of mutations that diminish IGF-1 in serum.
Across a panel of 21 tissues, we used RT-PCR to evaluate the effects of the PappA (−/−)
mutation on expression of Igfbp5, which served as an in vivo indicator of IGF-1 signaling …
localized IGF-1 bioavailability and a 30% increase in mean life span. However, it is
uncertain which tissues exhibit reduced IGF-1 signals in the PappA (−/−) mouse, and
whether effects of this mutation parallel those of mutations that diminish IGF-1 in serum.
Across a panel of 21 tissues, we used RT-PCR to evaluate the effects of the PappA (−/−)
mutation on expression of Igfbp5, which served as an in vivo indicator of IGF-1 signaling …