Observations on the growth in vitro of myeloid progenitor cells and fibroblasts from hemizygotes and heterozygotes for “complete” and “partial” hypoxanthine-guanine …
RO McKeran, A Howell, TM Andrews… - Journal of the …, 1974 - Elsevier
(1) Brain and bone marrow are similar, in that they both depend heavily on the purine
salvage pathways (catalysed by purine phosphoribosyltransferases), rather than on de novo
purine biosynthesis for the supply of ribonucleotides. 2.(2) Myeloid progenitor cells from
affected hemizygotes with either the complete, or partial deficiency of hypoxanthine guanine
phosphoribosyltransferase (HGPRT) produced fewer, and in the complete deficiency,
smaller cell colonies than normal controls when cultured in agar. Conversely, the ability of …
salvage pathways (catalysed by purine phosphoribosyltransferases), rather than on de novo
purine biosynthesis for the supply of ribonucleotides. 2.(2) Myeloid progenitor cells from
affected hemizygotes with either the complete, or partial deficiency of hypoxanthine guanine
phosphoribosyltransferase (HGPRT) produced fewer, and in the complete deficiency,
smaller cell colonies than normal controls when cultured in agar. Conversely, the ability of …