Use of dimedone-based chemical probes for sulfenic acid detection: evaluation of conditions affecting probe incorporation into redox-sensitive proteins

C Klomsiri, KJ Nelson, E Bechtold, L Soito… - Methods in …, 2010 - Elsevier
Sulfenic acids, formed as transient intermediates during the reaction of cysteine residues
with peroxides, play significant roles in enzyme catalysis and regulation, and are also
involved in the redox regulation of transcription factors and other signaling proteins.
Therefore, interest in the identification of protein sulfenic acids has grown substantially in the
past few years. Dimedone, which specifically traps sulfenic acids, has provided the basis for
the synthesis of a novel group of compounds that derivatize 1, 3-cyclohexadione, a …