Culturing of human nasal epithelial cells at the air liquid interface

L Müller, LE Brighton, JL Carson, WA Fischer II… - JoVE (Journal of …, 2013 - jove.com
In vitro models using human primary epithelial cells are essential in understanding key
functions of the respiratory epithelium in the context of microbial infections or inhaled agents.
Direct comparisons of cells obtained from diseased populations allow us to characterize
different phenotypes and dissect the underlying mechanisms mediating changes in
epithelial cell function. Culturing epithelial cells from the human tracheobronchial region has
been well documented, but is limited by the availability of human lung tissue or invasiveness …