Optimization of laser-capture microdissection for the isolation of enteric ganglia from fresh-frozen human tissue
AA May-Zhang, KK Deal… - JoVE (Journal of Visualized …, 2018 - jove.com
The purpose of this method is to obtain high-integrity RNA samples from enteric ganglia
collected from unfixed, freshly-resected human intestinal tissue using laser capture
microdissection (LCM). We have identified five steps in the workflow that are crucial for
obtaining RNA isolates from enteric ganglia with sufficiently high quality and quantity for
RNA-seq. First, when preparing intestinal tissue, each sample must have all excess liquid
removed by blotting prior to flattening the serosa as much as possible across the bottom of …
collected from unfixed, freshly-resected human intestinal tissue using laser capture
microdissection (LCM). We have identified five steps in the workflow that are crucial for
obtaining RNA isolates from enteric ganglia with sufficiently high quality and quantity for
RNA-seq. First, when preparing intestinal tissue, each sample must have all excess liquid
removed by blotting prior to flattening the serosa as much as possible across the bottom of …
