[HTML][HTML] High-efficiency FLP and ΦC31 site-specific recombination in mammalian cells
CS Raymond, P Soriano - PloS one, 2007 - journals.plos.org
PloS one, 2007•journals.plos.org
DNA site-specific recombinases (SSRs) such as Cre, FLPe, and φC31, are powerful tools for
analyzing gene function in vertebrates. While the availability of multiple high-efficiency SSRs
would facilitate a wide array of genomic engineering possibilities, efficient recombination in
mammalian cells has only been observed with Cre recombinase. Here we report the de
novo synthesis of mouse codon-optimized FLP (FLPo) and ΦC31 (ΦC31o) SSRs, which
result in recombination efficiencies similar to Cre.
analyzing gene function in vertebrates. While the availability of multiple high-efficiency SSRs
would facilitate a wide array of genomic engineering possibilities, efficient recombination in
mammalian cells has only been observed with Cre recombinase. Here we report the de
novo synthesis of mouse codon-optimized FLP (FLPo) and ΦC31 (ΦC31o) SSRs, which
result in recombination efficiencies similar to Cre.
DNA site-specific recombinases (SSRs) such as Cre, FLPe, and φC31, are powerful tools for analyzing gene function in vertebrates. While the availability of multiple high-efficiency SSRs would facilitate a wide array of genomic engineering possibilities, efficient recombination in mammalian cells has only been observed with Cre recombinase. Here we report the de novo synthesis of mouse codon-optimized FLP (FLPo) and ΦC31 (ΦC31o) SSRs, which result in recombination efficiencies similar to Cre.
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