[HTML][HTML] Method for controlled mitochondrial perturbation during phosphorus MRS in children
Medicine and science in sports and exercise, 2014•ncbi.nlm.nih.gov
Methods Data were collected from 66 sedentary 12–20 year-olds. Plantar flexion-volitional
MVC was assessed using a MRI-compatible exercise treadle device. MCSA of the calf
muscles were measured from MRI images. Data from the first 26 participants were utilized to
model the relationship between MVC and MCSA (predicted MVC= 24.763+ 0.0047* MCSA).
This model was then applied to the subsequent 40 participants. Results Volitional vs. model-
predicted mean MVC was 43.9±0.8 kg vs. 44.2±1.81 (P= 0.90). 31 P-MRS results when …
MVC was assessed using a MRI-compatible exercise treadle device. MCSA of the calf
muscles were measured from MRI images. Data from the first 26 participants were utilized to
model the relationship between MVC and MCSA (predicted MVC= 24.763+ 0.0047* MCSA).
This model was then applied to the subsequent 40 participants. Results Volitional vs. model-
predicted mean MVC was 43.9±0.8 kg vs. 44.2±1.81 (P= 0.90). 31 P-MRS results when …
Methods
Data were collected from 66 sedentary 12–20 year-olds. Plantar flexion-volitional MVC was assessed using a MRI-compatible exercise treadle device. MCSA of the calf muscles were measured from MRI images. Data from the first 26 participants were utilized to model the relationship between MVC and MCSA (predicted MVC= 24.763+ 0.0047* MCSA). This model was then applied to the subsequent 40 participants.
Results
Volitional vs. model-predicted mean MVC was 43.9±0.8 kg vs. 44.2±1.81 (P= 0.90). 31 P-MRS results when predicted and volitional MVC were similar showed expected changes during volitional MVC-based exercise. In contrast, volitional MVC was markedly lower than predicted in 4 participants, and produced minimal metabolic perturbation. Upon repeat testing, these individuals could perform their predicted MVC with coaching, which produced expected metabolic perturbations.
Conclusions
Compared to using MVC testing alone, utilizing MRI to predict muscle strength allows for a more accurate and standardized 31 P-MRS protocol during exercise in children. This method overcomes a major obstacle in assessing mitochondrial function in youth. These studies have importance as we seek to determine the role of mitochondrial function in youth with IR and diabetes and response to interventions.
ncbi.nlm.nih.gov