Tyrosine hydroxylase expression is unstable in a human immortalized mesencephalic cell line—studies in vitro and after intracerebral grafting in vivo
G Paul, NS Christophersen, H Raymon, C Kiaer… - Molecular and Cellular …, 2007 - Elsevier
We have studied the stability of the dopaminergic phenotype in a conditionally immortalized
human mesencephalic cell line, MESC2. 10. Even though MESC2. 10 cells exhibit features
of dopaminergic neurons in vitro, none of the cells expressed tyrosine hydroxylase (TH) after
transplantation into a rat model of Parkinson's disease. We examined whether this is caused
by cell death or loss of transmitter phenotype. Cells were cultured in differentiation medium,
then harvested and replated into the same medium where they continued to express TH …
human mesencephalic cell line, MESC2. 10. Even though MESC2. 10 cells exhibit features
of dopaminergic neurons in vitro, none of the cells expressed tyrosine hydroxylase (TH) after
transplantation into a rat model of Parkinson's disease. We examined whether this is caused
by cell death or loss of transmitter phenotype. Cells were cultured in differentiation medium,
then harvested and replated into the same medium where they continued to express TH …
