Reliable and high efficiency extraction of kidney immune cells

R Nistala, A Meuth, C Smith, A Annayya - JoVE (Journal of Visualized …, 2016 - jove.com
Immune system activation occurs in multiple kidney diseases and pathophysiological
processes. The immune system consists of both adaptive and innate components and
multiple cell types. Sometimes, the cell type of interest is present in very low numbers among
the large numbers of total cells isolated from the kidney. Hence, reliable and efficient
isolation of kidney mononuclear cell populations is important in order to study the
immunological problems associated with kidney diseases. Traditionally, tissue isolation of …