Background Three different cell types constitute the glomerular filter: mesangial cells,
endothelial cells, and podocytes. However, to what extent cellular heterogeneity exists
within healthy glomerular cell populations remains unknown. Methods We used nanodroplet-
based highly parallel transcriptional profiling to characterize the cellular content of purified
wild-type mouse glomeruli. Results Unsupervised clustering of nearly 13,000 single-cell
transcriptomes identified the three known glomerular cell types. We provide a …