Empowering multi-cohort gene expression analysis to increase reproducibility

WA Haynes, F Vallania, C Liu, E Bongen… - PACIFIC …, 2017 - World Scientific
PACIFIC SYMPOSIUM ON BIOCOMPUTING 2017, 2017World Scientific
A major contributor to the scientific reproducibility crisis has been that the results from
homogeneous, single-center studies do not generalize to heterogeneous, real world
populations. Multi-cohort gene expression analysis has helped to increase reproducibility by
aggregating data from diverse populations into a single analysis. To make the multi-cohort
analysis process more feasible, we have assembled an analysis pipeline which implements
rigorously studied meta-analysis best practices. We have compiled and made publicly …
A major contributor to the scientific reproducibility crisis has been that the results from homogeneous, single-center studies do not generalize to heterogeneous, real world populations. Multi-cohort gene expression analysis has helped to increase reproducibility by aggregating data from diverse populations into a single analysis. To make the multi-cohort analysis process more feasible, we have assembled an analysis pipeline which implements rigorously studied meta-analysis best practices. We have compiled and made publicly available the results of our own multi-cohort gene expression analysis of 103 diseases, spanning 615 studies and 36,915 samples, through a novel and interactive web application. As a result, we have made both the process of and the results from multi-cohort gene expression analysis more approachable for non-technical users.
World Scientific