High-definition spatial transcriptomics for in situ tissue profiling
Nature methods, 2019•nature.com
Spatial and molecular characteristics determine tissue function, yet high-resolution methods
to capture both concurrently are lacking. Here, we developed high-definition spatial
transcriptomics, which captures RNA from histological tissue sections on a dense, spatially
barcoded bead array. Each experiment recovers several hundred thousand transcript-
coupled spatial barcodes at 2-μm resolution, as demonstrated in mouse brain and primary
breast cancer. This opens the way to high-resolution spatial analysis of cells and tissues.
to capture both concurrently are lacking. Here, we developed high-definition spatial
transcriptomics, which captures RNA from histological tissue sections on a dense, spatially
barcoded bead array. Each experiment recovers several hundred thousand transcript-
coupled spatial barcodes at 2-μm resolution, as demonstrated in mouse brain and primary
breast cancer. This opens the way to high-resolution spatial analysis of cells and tissues.
Abstract
Spatial and molecular characteristics determine tissue function, yet high-resolution methods to capture both concurrently are lacking. Here, we developed high-definition spatial transcriptomics, which captures RNA from histological tissue sections on a dense, spatially barcoded bead array. Each experiment recovers several hundred thousand transcript-coupled spatial barcodes at 2-μm resolution, as demonstrated in mouse brain and primary breast cancer. This opens the way to high-resolution spatial analysis of cells and tissues.
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