Spatial organization of the somatosensory cortex revealed by osmFISH
Nature methods, 2018•nature.com
Global efforts to create a molecular census of the brain using single-cell transcriptomics are
producing a large catalog of molecularly defined cell types. However, spatial information is
lacking and new methods are needed to map a large number of cell type–specific markers
simultaneously on large tissue areas. Here, we describe a cyclic single-molecule
fluorescence in situ hybridization methodology and define the cellular organization of the
somatosensory cortex.
producing a large catalog of molecularly defined cell types. However, spatial information is
lacking and new methods are needed to map a large number of cell type–specific markers
simultaneously on large tissue areas. Here, we describe a cyclic single-molecule
fluorescence in situ hybridization methodology and define the cellular organization of the
somatosensory cortex.
Abstract
Global efforts to create a molecular census of the brain using single-cell transcriptomics are producing a large catalog of molecularly defined cell types. However, spatial information is lacking and new methods are needed to map a large number of cell type–specific markers simultaneously on large tissue areas. Here, we describe a cyclic single-molecule fluorescence in situ hybridization methodology and define the cellular organization of the somatosensory cortex.
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