[HTML][HTML] Expression of integrin alphavbeta6 in the intestinal epithelial cells of patients with inflammatory bowel disease
BS Feng, X Chen, P Li, PY Zheng, J Chong… - … American Journal of …, 2009 - ncbi.nlm.nih.gov
BS Feng, X Chen, P Li, PY Zheng, J Chong, DB Cho, SH He, SG Tang, PC Yang
North American Journal of Medical Sciences, 2009•ncbi.nlm.nih.govMaterials and Methods: Colonic biopsy was obtained from a group of IBD patients. The
expression of αvβ6 in the intestinal mucosa was detected by Western blotting. Human
colonic epithelial cell line T84 cells were stimulated by microbial antigen flagellin. The
expression of αvβ6 in T84 cells was evaluated by quantitative RT-PCR and Western blotting.
Results: The levels of αvβ6 in the intestinal mucosa were much lower than it in normal
control subjects. The serum levels of myeloperoxidase (MPO) were higher in IBD patients …
expression of αvβ6 in the intestinal mucosa was detected by Western blotting. Human
colonic epithelial cell line T84 cells were stimulated by microbial antigen flagellin. The
expression of αvβ6 in T84 cells was evaluated by quantitative RT-PCR and Western blotting.
Results: The levels of αvβ6 in the intestinal mucosa were much lower than it in normal
control subjects. The serum levels of myeloperoxidase (MPO) were higher in IBD patients …
Materials and Methods:
Colonic biopsy was obtained from a group of IBD patients. The expression of αvβ6 in the intestinal mucosa was detected by Western blotting. Human colonic epithelial cell line T84 cells were stimulated by microbial antigen flagellin. The expression of αvβ6 in T84 cells was evaluated by quantitative RT-PCR and Western blotting.
Results:
The levels of αvβ6 in the intestinal mucosa were much lower than it in normal control subjects. The serum levels of myeloperoxidase (MPO) were higher in IBD patients that were negatively correlated with the levels of αvβ6 in the intestinal mucosa. The expression of αvβ6 was detectable in T84 cells at naοve status that could be upregulated by exposure to microbial antigen flagellin. Pretreatment with MPO dramatically suppressed the expression of αvβ6 in T84 cells.
Conclusions:
We conclude that the expression of αvβ6 was suppressed in IBD intestinal mucosa, which could be resulted from the high levels of MPO.
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