[HTML][HTML] Expressing the human proteome for affinity proteomics: optimising expression of soluble protein domains and in vivo biotinylation
T Keates, CDO Cooper, P Savitsky, CK Allerston… - New biotechnology, 2012 - Elsevier
The generation of affinity reagents to large numbers of human proteins depends on the
ability to express the target proteins as high-quality antigens. The Structural Genomics
Consortium (SGC) focuses on the production and structure determination of human proteins.
In a 7-year period, the SGC has deposited crystal structures of> 800 human protein
domains, and has additionally expressed and purified a similar number of protein domains
that have not yet been crystallised. The targets include a diversity of protein domains, with …
ability to express the target proteins as high-quality antigens. The Structural Genomics
Consortium (SGC) focuses on the production and structure determination of human proteins.
In a 7-year period, the SGC has deposited crystal structures of> 800 human protein
domains, and has additionally expressed and purified a similar number of protein domains
that have not yet been crystallised. The targets include a diversity of protein domains, with …