STAT1 is required for iNOS activation, but not IL-6 production in murine fibroblasts
T Samardzic, V Jankovic, S Stosic-Grujicic, V Trajkovic - Cytokine, 2001 - Elsevier
T Samardzic, V Jankovic, S Stosic-Grujicic, V Trajkovic
Cytokine, 2001•ElsevierThe role of transcription factor STAT1 in production of pro-inflammatory mediators nitric
oxide (NO) and IL-6 was examined in murine embryonic fibroblasts. While cells from wild-
type animals released large amounts of NO after stimulation with IFN-γ in combination with
LPS, TNF-α or IL-1, their STAT1-deficient counterparts failed to synthesise detectable levels
of this free radical gas. Inability of STAT1-/-fibroblasts to produce NO was accompanied by
complete absence of mRNA for iNOS and its transcription factor IRF-1, both readily …
oxide (NO) and IL-6 was examined in murine embryonic fibroblasts. While cells from wild-
type animals released large amounts of NO after stimulation with IFN-γ in combination with
LPS, TNF-α or IL-1, their STAT1-deficient counterparts failed to synthesise detectable levels
of this free radical gas. Inability of STAT1-/-fibroblasts to produce NO was accompanied by
complete absence of mRNA for iNOS and its transcription factor IRF-1, both readily …
The role of transcription factor STAT1 in production of pro-inflammatory mediators nitric oxide (NO) and IL-6 was examined in murine embryonic fibroblasts. While cells from wild-type animals released large amounts of NO after stimulation with IFN-γ in combination with LPS, TNF-α or IL-1, their STAT1-deficient counterparts failed to synthesise detectable levels of this free radical gas. Inability of STAT1-/- fibroblasts to produce NO was accompanied by complete absence of mRNA for iNOS and its transcription factor IRF-1, both readily upregulated in wild-type cells. However, treatment with cytokines (IFN-γ, TNF-α, IL-1, IL-17) significantly increased IL-6 generation in STAT1-deficient fibroblasts. These results indicate that STAT1 activation and subsequent IRF-1 transcription are required for induction of iNOS, but not IL-6 in murine fibroblasts.
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