Increased neutrophil oxidative burst metabolism in systemic lupus erythematosus

SF Perazzio, R Salomão, NP Silva, LEC Andrade - Lupus, 2012 - journals.sagepub.com
SF Perazzio, R Salomão, NP Silva, LEC Andrade
Lupus, 2012journals.sagepub.com
Introduction: There is increased frequency of discoid lesions (2.7%) and SLE (0.5%) in
patients with chronic granulomatosus disease, but the literature is still controversial about
phagocyte oxidative burst in SLE patients. Materials and methods: 300 SLE patients and
301 blood donors were evaluated for quantitation of the oxidative burst in phagocytes by
flow cytometry based on the oxidation of 2, 7-dichlorofluorescein-diacetate after stimuli with
Staphylococcus aureus and Pseudomonas aeruginosa. Results: Neutrophils from SLE …
Introduction
There is increased frequency of discoid lesions (2.7%) and SLE (0.5%) in patients with chronic granulomatosus disease, but the literature is still controversial about phagocyte oxidative burst in SLE patients.
Materials and methods
300 SLE patients and 301 blood donors were evaluated for quantitation of the oxidative burst in phagocytes by flow cytometry based on the oxidation of 2,7-dichlorofluorescein-diacetate after stimuli with Staphylococcus aureus and Pseudomonas aeruginosa.
Results
Neutrophils from SLE patients displayed higher basal reactive oxygen species (ROS) production than healthy controls [Mean of fluorescence intensity (MFI) = 53.77 ± 11.38 vs 15.08 ± 2.63, p < 0.001] and after stimulation with S. aureus (MFI = 355.46 ± 58.55 vs 151.92 ± 28.25, p < 0.001) or P. aeruginosa (MFI = 82.53 ± 10.1 vs 48.99 ± 6.74, p < 0.001). There was stronger neutrophil response after bacterial stimuli (ΔMFI) in SLE patients than in healthy controls (S. aureus = 301.69 ± 54.42 vs 118.38 ± 26.03, p < 0.001; P. aeruginosa = 28.76 ± 12.3 vs 15.45 ± 5.15, p < 0.001), but no difference with respect to the oxidative burst profile according to disease activity (SLEDAI ≥ 6) or severity (SLICC-DI ≥2). Patients with kidney involvement presented higher basal and stimulated ROS production in neutrophils.
Discussion
The present findings corroborate the important role of innate immunity in SLE and implicate neutrophils in the pathophysiology of the disease.
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