Expression and distribution of InsP3 receptor subtypes in proliferating vascular smooth muscle cells

PN Tasker, CW Taylor, GF Nixon - Biochemical and biophysical research …, 2000 - Elsevier
PN Tasker, CW Taylor, GF Nixon
Biochemical and biophysical research communications, 2000Elsevier
The expression and distribution of types 1, 2, and 3 inositol 1, 4, 5-trisphosphate receptor
(InsP3R) in proliferating, primary cultures of rat aortic smooth muscle were compared to fully
developed and differentiated rat aortic smooth muscle. Subtype-specific InsP3R antibodies
revealed that the expression of type 1 InsP3R was similar in cultured aortic cells and aorta
homogenate but expression of type 2 and 3 InsP3R subtypes was increased 3-fold in
cultured aortic cells. The distribution of the type 1 InsP3R was located throughout the …
The expression and distribution of types 1, 2, and 3 inositol 1,4,5-trisphosphate receptor (InsP3R) in proliferating, primary cultures of rat aortic smooth muscle were compared to fully developed and differentiated rat aortic smooth muscle. Subtype-specific InsP3R antibodies revealed that the expression of type 1 InsP3R was similar in cultured aortic cells and aorta homogenate but expression of type 2 and 3 InsP3R subtypes was increased 3-fold in cultured aortic cells. The distribution of the type 1 InsP3R was located throughout the cytoplasm; type 2 InsP3R was found closely associated with the nucleus and at the plasma membrane; type 3 InsP3R was distributed predominantly around the nucleus. Alterations in InsP3R subtype expression and localization may have important functions in regulating intracellular calcium release around the nucleus when vascular smooth muscle cells switch to a more proliferating phenotype.
Elsevier