SUnSET, a nonradioactive method to monitor protein synthesis

EK Schmidt, G Clavarino, M Ceppi, P Pierre - Nature methods, 2009 - nature.com
EK Schmidt, G Clavarino, M Ceppi, P Pierre
Nature methods, 2009nature.com
We developed a nonradioactive fluorescence-activated cell sorting–based assay, called
surface sensing of translation (SUnSET), which allows the monitoring and quantification of
global protein synthesis in individual mammalian cells and in heterogeneous cell
populations. We demonstrate here, using mouse dendritic and T cells as a model, that
SUnSET offers a technical alternative to classical radioactive labeling methods for the study
of mRNA translation and cellular activation.
Abstract
We developed a nonradioactive fluorescence-activated cell sorting–based assay, called surface sensing of translation (SUnSET), which allows the monitoring and quantification of global protein synthesis in individual mammalian cells and in heterogeneous cell populations. We demonstrate here, using mouse dendritic and T cells as a model, that SUnSET offers a technical alternative to classical radioactive labeling methods for the study of mRNA translation and cellular activation.
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