Lipid transfer inhibitor protein (apolipoprotein F) concentration in normolipidemic and hyperlipidemic subjects
RE Morton, HM Gnizak, DJ Greene, KH Cho… - Journal of lipid …, 2008 - jlr.org
Lipid transfer inhibitor protein (LTIP) is an important regulator of cholesteryl ester transfer
protein function. We report the development of an immunoassay for LTIP and its use to
quantify LTIP in plasma of varying lipid contents. A rabbit antibody against bacterially
produced recombinant LTIP detected two LTIP isoforms in plasma differing in carbohydrate
content. This antibody was used in a competitive, enzyme-linked immunoassay that uses
partially purified LTIP bound to microtiter plates. To optimize LTIP immunoreactivity, plasma …
protein function. We report the development of an immunoassay for LTIP and its use to
quantify LTIP in plasma of varying lipid contents. A rabbit antibody against bacterially
produced recombinant LTIP detected two LTIP isoforms in plasma differing in carbohydrate
content. This antibody was used in a competitive, enzyme-linked immunoassay that uses
partially purified LTIP bound to microtiter plates. To optimize LTIP immunoreactivity, plasma …