ApoF knockdown increases cholesteryl ester transfer to LDL and impairs cholesterol clearance in fat-fed hamsters
RE Morton, Y Liu, L Izem - Journal of lipid research, 2019 - jlr.org
Cholesteryl ester transfer protein (CETP) regulates intravascular lipoprotein metabolism. In
vitro studies indicate that ApoF alters CETP function by inhibiting its activity with LDL. To
explore in vivo the complexities driving ApoF's effects on CETP, we developed a siRNA-
based hamster model of ApoF knockdown. In both male and female hamsters on chow-or fat-
fed diets, we measured lipoprotein levels and composition, determined CETP-mediated
transfer of cholesteryl esters (CEs) between lipoproteins, and quantified reverse cholesterol …
vitro studies indicate that ApoF alters CETP function by inhibiting its activity with LDL. To
explore in vivo the complexities driving ApoF's effects on CETP, we developed a siRNA-
based hamster model of ApoF knockdown. In both male and female hamsters on chow-or fat-
fed diets, we measured lipoprotein levels and composition, determined CETP-mediated
transfer of cholesteryl esters (CEs) between lipoproteins, and quantified reverse cholesterol …
