De novo fatty acid synthesis by freshly isolated alveolar type II epithelial cells
WM Maniscalco, JN Finkelstein, AB Parkhurst - Biochimica et Biophysica …, 1983 - Elsevier
Fatty acid synthesis was studied in freshly isolated type II pneuniocytes from rabbits by 3 H 2
O and (U-14 C)-labeled glucose, lactate and pyruvate incorporation and the activity of acetyl-
CoA carboxylase. The rate of lactate incorporation into fatty acids was 3-fold greater than
glucose incorporation; lactate incorporation into the glycerol portion of lipids was very low
but glucose incorporation into this fraction was approximately equal to incorporation into
fatty acids. The highest rate of de novo fatty acid synthesis (3 H 2 O incorporation) required …
O and (U-14 C)-labeled glucose, lactate and pyruvate incorporation and the activity of acetyl-
CoA carboxylase. The rate of lactate incorporation into fatty acids was 3-fold greater than
glucose incorporation; lactate incorporation into the glycerol portion of lipids was very low
but glucose incorporation into this fraction was approximately equal to incorporation into
fatty acids. The highest rate of de novo fatty acid synthesis (3 H 2 O incorporation) required …