Immunogenicity and antigenicity of Plasmodium vivax merozoite surface protein 10

Y Cheng, B Wang, J Sattabongkot, CS Lim… - Parasitology …, 2014 - Springer
Y Cheng, B Wang, J Sattabongkot, CS Lim, T Tsuboi, ET Han
Parasitology research, 2014Springer
Among the proteins involved in the invasion by merozoite, the glycosylphosphatidylinositol-
anchored proteins (GPI-APs) are suggested as potential vaccine candidates because of
their localization to apical organelles and the surface; these candidates are predicted to play
essential roles during invasion. As a GPI-AP, Plasmodium vivax merozoite surface protein
10 (PvMSP-10) induces high antibody titers. However, such high antibody titers have shown
no protective efficacy for animals challenged with P. vivax parasites in a previous study. To …
Abstract
Among the proteins involved in the invasion by merozoite, the glycosylphosphatidylinositol-anchored proteins (GPI-APs) are suggested as potential vaccine candidates because of their localization to apical organelles and the surface; these candidates are predicted to play essential roles during invasion. As a GPI-AP, Plasmodium vivax merozoite surface protein 10 (PvMSP-10) induces high antibody titers. However, such high antibody titers have shown no protective efficacy for animals challenged with P. vivax parasites in a previous study. To adequately evaluate the immunogenicity and further characterize PvMSP-10 in order to understand its vaccine potential, we assessed its immunogenicity by immunizing BALB/c mice with cell-free expressed recombinant PvMSP-10 protein. The antigenicity of MSP-10 was analyzed, and we found 42 % sensitivity and 95 % specificity using serum samples from P. vivax-infected Korean patients. The IgG1 and IgG3 were the predominant immunoreactive antibodies against PvMSP-10 in vivax patient sera, and IgG1 and IgG3 and Th1-type cytokines were predominantly secreted in PvMSP-10-immunized mice. We conclude that the immunogenicity and antigenicity of MSP-10 may serve as a potential vaccine against vivax malaria.
Springer