An efficient sieving method to isolate intact glomeruli from adult rat kidney

BM Rush, SA Small, DB Stolz, RJ Tan - JoVE (Journal of Visualized …, 2018 - jove.com
Preservation of glomerular structure and function is pivotal in the prevention of
glomerulonephritis, a category of kidney disease characterized by proteinuria which can
eventually lead to chronic and end-stage renal disease. The glomerulus is a complex
apparatus responsible for the filtration of plasma from the body. In disease, structural
integrity is lost and allows for the abnormal leakage of plasma contents into the urine. A
method to isolate and examine glomeruli in culture is critical for the study of these diseases …