[HTML][HTML] Hepatotoxin-induced changes in the adult murine liver promote MYC-induced tumorigenesis

S Beer, K Komatsubara, DI Bellovin, M Kurobe… - PloS one, 2008 - journals.plos.org
S Beer, K Komatsubara, DI Bellovin, M Kurobe, K Sylvester, DW Felsher
PloS one, 2008journals.plos.org
Background Overexpression of the human c-MYC (MYC) oncogene is one of the most
frequently implicated events in the pathogenesis of hepatocellular carcinoma (HCC).
Previously, we have shown in a conditional transgenic mouse model that MYC
overexpression is restrained from inducing mitotic cellular division and tumorigenesis in the
adult liver; whereas, in marked contrast, MYC induces robust proliferation associated with
the very rapid onset of tumorigenesis in embryonic and neonatal mice …
Background
Overexpression of the human c-MYC (MYC) oncogene is one of the most frequently implicated events in the pathogenesis of hepatocellular carcinoma (HCC). Previously, we have shown in a conditional transgenic mouse model that MYC overexpression is restrained from inducing mitotic cellular division and tumorigenesis in the adult liver; whereas, in marked contrast, MYC induces robust proliferation associated with the very rapid onset of tumorigenesis in embryonic and neonatal mice.
Methodology/Principal Findings
Here, we show that non-genotoxic hepatotoxins induce changes in the liver cellular context associated with increased cellular proliferation and enhanced tumorigenesis. Both 5-diethoxycarbonyl-1,4-dihydrocollidine (DDC) and carbon tetrachloride (CCl4) cooperate with MYC to greatly accelerate the onset of liver cancer in an adult host to less than 7 days versus a mean latency of onset of over 35 weeks for MYC alone. These hepatotoxin-enhanced liver tumors grossly and histologically resemble embryonic and neonatal liver tumors. Importantly, we found that MYC overexpression is only capable of inducing expression of the mitotic Cyclin B1 in embryonic/neonatal hosts or adult hosts that were treated with either carcinogen.
Conclusion/Significance
Our results suggest a model whereby oncogenes can remain latently activated, but exposure of the adult liver to hepatotoxins that promote hepatocyte proliferation can rapidly uncover their malignant potential.
PLOS