Although the role of astrocyte glutamate transporters in glutamate clearance is well
illustrated, the role of glutamine synthetase (GS) that influences this process remains to be
elucidated. We examined whether GS affected the uptake of glutamate in astrocytes in vitro.
The glutamate uptake was assessed by measuring the concentration of glutamate and
glutamine in culture medium in the presence or absence of glutamate. We demonstrated that
inhibition of GS in astrocytes by MSO significantly impaired glutamate uptake and glutamine …