Early embryonic lethality in mice deficient in the p110β catalytic subunit of PI 3-kinase.
L Bi, I Okabe, DJ Bernard, RL Nussbaum - 2002 - cabidigitallibrary.org
L Bi, I Okabe, DJ Bernard, RL Nussbaum
2002•cabidigitallibrary.orgThis article presents the successful targeting of the Pik3cb encoding the beta isoform of
p110, to create a partial deletion allele which, in the homozygous state, leads to very early
embryonic death. Genotyping results of embryos at different stages of embryonic
development indicated that the deficiency of homozygous embryos was occurring very early
in development. Zygotes with the Pik3cb del/del genotype were nonviable very early after
fertilization, leading to a deficiency of such embryos even at the blastocyst stage. Not only is …
p110, to create a partial deletion allele which, in the homozygous state, leads to very early
embryonic death. Genotyping results of embryos at different stages of embryonic
development indicated that the deficiency of homozygous embryos was occurring very early
in development. Zygotes with the Pik3cb del/del genotype were nonviable very early after
fertilization, leading to a deficiency of such embryos even at the blastocyst stage. Not only is …
Abstract
This article presents the successful targeting of the Pik3cb encoding the beta isoform of p110, to create a partial deletion allele which, in the homozygous state, leads to very early embryonic death. Genotyping results of embryos at different stages of embryonic development indicated that the deficiency of homozygous embryos was occurring very early in development. Zygotes with the Pik3cbdel/del genotype were nonviable very early after fertilization, leading to a deficiency of such embryos even at the blastocyst stage. Not only is expression of one allele of each isoform sufficient for normal development, but also it is sufficient even when the other isoform is also haploinsufficient.
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