[HTML][HTML] High level expression of recombinant porcine coagulation factor VIII
CB Doering, JF Healey, ET Parker, RT Barrow… - Journal of Biological …, 2002 - ASBMB
Recombinant human factor VIII expression levels, in vitro and in vivo, are significantly lower
than levels obtained for other recombinant coagulation proteins. Here we describe the
generation, high level expression and characterization of a recombinant B-domain-deleted
porcine factor VIII molecule. Recombinant B-domain-deleted porcine factor VIII expression
levels are 10-to 14-fold greater than recombinant B-domain-deleted human factor VIII levels
by transient and stable expression in multiple cell lines. Peak expression of 140 units· 10 6 …
than levels obtained for other recombinant coagulation proteins. Here we describe the
generation, high level expression and characterization of a recombinant B-domain-deleted
porcine factor VIII molecule. Recombinant B-domain-deleted porcine factor VIII expression
levels are 10-to 14-fold greater than recombinant B-domain-deleted human factor VIII levels
by transient and stable expression in multiple cell lines. Peak expression of 140 units· 10 6 …