The viability of autologous human red cells stored in additive solution 5 and exposed to 25 C for 24 hours

TJ Reid, JG Babcock, CP Derse‐Anthony, HR Hill… - …, 1999 - Wiley Online Library
TJ Reid, JG Babcock, CP Derse‐Anthony, HR Hill, LE Lippert, JR Hess
Transfusion, 1999Wiley Online Library
BACKGROUND: No data exist on the viability of red cells (RBCs) storsed in modern additive
solution systems and allowed to warm above 10° C. STUDY DESIGN AND METHODS: In a
randomized crossover study, 3 units of blood were collected at least 8 weeks apart from 11
volunteer donors and stored in additive solution 5 (AS‐5). Of 3 units from each volunteer, 1
was stored for 6 weeks at 4° C, 1 for 5 weeks at 4° C except for 24 hours at 25° C on Day 14,
and 1 for 5 weeks at 4° C except for 24 hours at 25° C on Day 28. Units were sampled …
BACKGROUND: No data exist on the viability of red cells (RBCs) storsed in modern additive solution systems and allowed to warm above 10°C.
STUDY DESIGN AND METHODS: In a randomized crossover study, 3 units of blood were collected at least 8 weeks apart from 11 volunteer donors and stored in additive solution 5 (AS‐5). Of 3 units from each volunteer, 1 was stored for 6 weeks at 4°C, 1 for 5 weeks at 4°C except for 24 hours at 25°C on Day 14, and 1 for 5 weeks at 4°C except for 24 hours at 25°C on Day 28. Units were sampled periodically during storage; at the end of storage, viability was measured by the 99mTc/51Cr double‐label method.
RESULTS: RBC viability was not significantly different in the storage protocols. Less than 1 percent of stored cells hemolyzed. RBC ATP concentrations at the end of storage correlated with viability and were approximately equal in the warmed units after 30 days' storage and the conventionally stored units after 42 days.
CONCLUSIONS: The data suggest that RBCs stored in AS‐5 and allowed to warm to 25°C for 24 hours lose about 12 days of their shelf life.
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