1H-Observe/13C-decouple spectroscopic measurements of lactate and glutamate in the rat brain in vivo.
DL Rothman, KL Behar… - Proceedings of the …, 1985 - National Acad Sciences
Proceedings of the National Academy of Sciences, 1985•National Acad Sciences
We have used (13C)-1H NMR spectroscopy at 360.13 MHz to resolve the 13C coupled
proton resonance of glutamate and lactate in the rat brain in vivo. The time required for the
13C fractional enrichment of the 4-CH2 position of brain glutamate to reach isotopic steady
state was determined during a continuous infusion of D-[1-13C] glucose. Under conditions of
ischemia, measurements made of the 3-CH3 of lactate in (13C)-1H NMR spectra revealed
the relative contribution of brain glucose and glycogen to lactate formation.(13C)-1H NMR …
proton resonance of glutamate and lactate in the rat brain in vivo. The time required for the
13C fractional enrichment of the 4-CH2 position of brain glutamate to reach isotopic steady
state was determined during a continuous infusion of D-[1-13C] glucose. Under conditions of
ischemia, measurements made of the 3-CH3 of lactate in (13C)-1H NMR spectra revealed
the relative contribution of brain glucose and glycogen to lactate formation.(13C)-1H NMR …
We have used (13C)-1H NMR spectroscopy at 360.13 MHz to resolve the 13C coupled proton resonance of glutamate and lactate in the rat brain in vivo. The time required for the 13C fractional enrichment of the 4-CH2 position of brain glutamate to reach isotopic steady state was determined during a continuous infusion of D-[1-13C]glucose. Under conditions of ischemia, measurements made of the 3-CH3 of lactate in (13C)-1H NMR spectra revealed the relative contribution of brain glucose and glycogen to lactate formation. (13C)-1H NMR was 11 times more sensitive than 13C NMR for the detection of 13C in the 3-CH3 position of lactate and 6 times more sensitive for the detection of 13C in the 4-CH2 of glutamate under similar in vivo conditions.
National Acad Sciences