Targeted lipidomics using electron capture atmospheric pressure chemical ionization mass spectrometry

SH Lee, MV Williams, RN DuBois… - … in Mass Spectrometry, 2003 - Wiley Online Library
SH Lee, MV Williams, RN DuBois, IA Blair
Rapid Communications in Mass Spectrometry, 2003Wiley Online Library
There is an increasing need to be able to conduct quantitative lipidomics analyses as a
complement to proteomics studies. The highest specificity for proteomics analysis can be
obtained using methodology based on electrospray ionization (ESI) or atmospheric pressure
chemical ionization (APCI) coupled with liquid chromatography/tandem mass spectrometry
(LC/MS/MS). For lipidomics analysis it is often necessary to be able to separate enantiomers
and regioisomers. This can be very challenging when using methodology based on …
Abstract
There is an increasing need to be able to conduct quantitative lipidomics analyses as a complement to proteomics studies. The highest specificity for proteomics analysis can be obtained using methodology based on electrospray ionization (ESI) or atmospheric pressure chemical ionization (APCI) coupled with liquid chromatography/tandem mass spectrometry (LC/MS/MS). For lipidomics analysis it is often necessary to be able to separate enantiomers and regioisomers. This can be very challenging when using methodology based on conventional reversed‐phase chromatography. Normal‐phase chromatography using chiral columns can provide dramatic improvements in the resolution of enantiomers and regioisomers. However, conventional ESI‐ and APCI‐MS/MS has limited sensitivity, which makes it difficult to conduct studies in cell culture systems where only trace amounts of non‐esterified bioactive lipids are present. The use of electron capture APCI‐MS/MS overcomes this problem. Enantiomers and regioisomers of diverse bioactive lipids can be quantified using stable isotope dilution methodology coupled with normal‐phase chiral chromatography and electron capture APCI‐MS/MS. This methodology has allowed a lipidomics profile from rat epithelial cells maintained in culture to be delineated and allowed the effect of a non‐selective lipoxygenase inhibitor to be assessed. Copyright © 2003 John Wiley & Sons, Ltd.
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