[PDF][PDF] Biochemical and pharmacological characterization of the cyclooxygenase activity of human blood prostaglandin endoperoxide synthases.

P Patrignani, MR Panara, A Greco, O Fusco… - … of Pharmacology and …, 1994 - Citeseer
P Patrignani, MR Panara, A Greco, O Fusco, C Natoli, S Iacobelli, F Cipollone, A Ganci…
Journal of Pharmacology and Experimental Therapeutics, 1994Citeseer
The aim of our study was to characterize a model of human prostaglandin endoperoxide
synthase-2(PGHS-2) expression allowing the assessment of pharmacological inhibition in
vitro and ex vivo. Heparinized human whole blood samples were incubated with
lipopolysaccharide(LPS, 0.1-50 pg/ml) for 0 to 24 hr at 37#{176} C. The contribution of
platelet PGHS-i was sup-pressed by either pretreating the subjects with aspirin (300 mg 48
hr before sampling) or adding aspirin (10 pg/ml) in vitro at time 0. PGE2 was measured by …
Abstract
The aim of our study was to characterize a model of human prostaglandin endoperoxide synthase-2(PGHS-2) expression allowing the assessment of pharmacological inhibition in vitro and ex vivo. Heparinized human whole blood samples were incubated with lipopolysaccharide(LPS, 0.1-50 pg/ml) for 0 to 24 hr at 37#{176} C. The contribution of platelet PGHS-i was sup-pressed by either pretreating the subjects with aspirin (300 mg
48 hr before sampling) or adding aspirin (10 pg/ml) in vitro at time 0. PGE2 was measured by radioimmunoassay. LPS in-duced expression of cyclooxygenase activity in a time-and concentration-dependent fashion. After 24 hr at 10 tg/ml LPS, PGE2 production averaged i2. ią6.2 ng/ml (meanąSD, n=
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